ADCY1 ELISA Kit

Produktnummer ABIN5651428

Produktdetails ADCY1 ELISA Kit

Target: ADCY1 (Adenylate Cyclase 1 (Brain) (ADCY1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.94 ng/mL - 60 ng/mL

Untere Nachweisgrenze: 0.94 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase 1, Brain (ADCY1). No significant cross-reactivity or interference between Adenylate Cyclase 1, Brain (ADCY1) and analogues was observed.

Sensitivität: 0.38 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 1, Brain (ADCY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 1, Brain (ADCY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 1, Brain (ADCY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylate Cyclase 1, Brain (ADCY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase 1, Brain (ADCY1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase 1, Brain (ADCY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu ADCY1

Target: ADCY1 (Adenylate Cyclase 1 (Brain) (ADCY1))

Andere Bezeichnung: Adenylate Cyclase 1, Brain (ADCY1 Produkte)

Synonyme: AC1 ELISA Kit, D11Bwg1392e ELISA Kit, I-AC ELISA Kit, brl ELISA Kit, mKIAA4070 ELISA Kit, Ac1 ELISA Kit, adenylate cyclase 1 ELISA Kit, adenylate cyclase type 1 ELISA Kit, adenylate cyclase 1 (brain) ELISA Kit, ADCY1 ELISA Kit, LOC100449504 ELISA Kit, adcy1 ELISA Kit, Adcy1 ELISA Kit

Hintergrund:

Gene Name: Adenylate Cyclase 1, Brain

Gene Aliases: AC1, ATP pyrophosphate-lyase 1, Ca(2+)/calmodulin-activated adenylyl cyclase, Adenylate cyclase type I

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma