APRT ELISA Kit

Produktnummer ABIN5651412

Produktdetails APRT ELISA Kit

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Adenine Phosphoribosyltransferase (APRT). No significant cross-reactivity or interference between Adenine Phosphoribosyltransferase (APRT) and analogues was observed.

Sensitivität: 0.061 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenine Phosphoribosyltransferase (APRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenine Phosphoribosyltransferase (APRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenine Phosphoribosyltransferase (APRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenine Phosphoribosyltransferase (APRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenine Phosphoribosyltransferase (APRT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenine Phosphoribosyltransferase (APRT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu APRT

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Andere Bezeichnung: Adenine Phosphoribosyltransferase (APRT Produkte)

Synonyme: Tb07.43M14.200 ELISA Kit, Tb07.43M14.180 ELISA Kit, C85684 ELISA Kit, AMP ELISA Kit, APRTD ELISA Kit, adenine phosphoribosyltransferase ELISA Kit, adenine phosphoribosyl transferase ELISA Kit, CND05020 ELISA Kit, Tb927.7.1790 ELISA Kit, Tb927.7.1780 ELISA Kit, Arnit_0941 ELISA Kit, Saut_1231 ELISA Kit, Fbal_1180 ELISA Kit, PH_RS07970 ELISA Kit, PF_RS08760 ELISA Kit, PAB_RS02560 ELISA Kit, Aprt ELISA Kit, APRT ELISA Kit

Hintergrund:

Gene Name: Adenine Phosphoribosyltransferase

Gene Aliases: AMP, APRTase

Gen-ID: 353

UniProt: P07741

Pathways: Ribonucleoside Biosynthetic Process