HMGCR ELISA Kit

Produktnummer ABIN5651276

Produktdetails HMGCR ELISA Kit

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). No significant cross-reactivity or interference between 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) and analogues was observed.

Sensitivität: 2.35 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu HMGCR

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Andere Bezeichnung: 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR Produkte)

Synonyme: HMGR ELISA Kit, LDLCQ3 ELISA Kit, hmg-coa-r ELISA Kit, HMG-CoAR ELISA Kit, Red ELISA Kit, 3H3M ELISA Kit, BcDNA:LD15354 ELISA Kit, CG10367 ELISA Kit, Clb ELISA Kit, DmHMG-CoAr ELISA Kit, Dmel\\CG10367 ELISA Kit, HMGCR ELISA Kit, HMGCR_Dm ELISA Kit, HMGCoAR ELISA Kit, HMGCoARr ELISA Kit, HMGCoAr ELISA Kit, HmG-CoAR ELISA Kit, Hmg ELISA Kit, bro1 ELISA Kit, bs32f05.y1 ELISA Kit, clb ELISA Kit, hmgc ELISA Kit, hmgcr ELISA Kit, hmgrc ELISA Kit, l(3)01152 ELISA Kit, l(3)04684 ELISA Kit, hmgcr2 ELISA Kit, im:6912716 ELISA Kit, zgc:110746 ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase L homeolog ELISA Kit, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ELISA Kit, HMG Coenzyme A reductase ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase b ELISA Kit, HMGCR ELISA Kit, hmgcr.L ELISA Kit, Hmgcr ELISA Kit, hmgcrb ELISA Kit

Hintergrund:

Gene Name: 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase

Gene Aliases: HMG-CoA Reductase, Hydroxymethylglutaryl-CoA Reductase

Pathways: AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha