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HSD11B2 ELISA Kit

HSD11B2 Reaktivität: Human Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5651254
  • Target Alle HSD11B2 ELISA Kits anzeigen
    HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))
    Reaktivität
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    78.12 pg/mL - 5000 pg/mL
    Untere Nachweisgrenze
    78.12 pg/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). No significant cross-reactivity or interference between 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) and analogues was observed.
    Sensitivität
    33 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle HSD11B2 ELISA Kits anzeigen
    HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))
    Andere Bezeichnung
    11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11B2 Produkte)
    Synonyme
    MGC81883 ELISA Kit, 11-HSD2 ELISA Kit, AME ELISA Kit, AME1 ELISA Kit, HSD11K ELISA Kit, HSD2 ELISA Kit, SDR9C3 ELISA Kit, 11HSD2 ELISA Kit, HSD11B2 ELISA Kit, hydroxysteroid (11-beta) dehydrogenase 2 L homeolog ELISA Kit, hydroxysteroid 11-beta dehydrogenase 2 ELISA Kit, hsd11b2.L ELISA Kit, HSD11B2 ELISA Kit, Hsd11b2 ELISA Kit
    Hintergrund

    Gene Name: 11-Beta-Hydroxysteroid Dehydrogenase Type 2

    Gene Aliases: AME, AME1, HSD11K, HSD2, SDR9C3, Short Chain Dehydrogenase/Reductase Family 9C,Member 3, NAD-dependent 11-beta-hydroxysteroid dehydrogenase

    Pathways
    Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones
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