1,25 Dihydroxy Vitamin D3 ELISA Kit

Produktnummer ABIN5651249

Diverse Spezies 1,25 Dihydroxy Vitamin D3 ELISA Kit, Colorimetric Assay für die Quantifizierung von Diverse Spezies 1,25 Dihydroxy Vitamin D3.

Kurzübersicht für 1,25 Dihydroxy Vitamin D3 ELISA Kit (ABIN5651249)

Target: 1,25 Dihydroxy Vitamin D3 (DHVD3) (1,25-Dihydroxy Vitamin D3 (DHVD3))

Reaktivität: Diverse Spezies

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 24.69 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails 1,25 Dihydroxy Vitamin D3 ELISA Kit

Untere Nachweisgrenze: 24.69 pg/mL

Verwendungszweck: 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit for detecting DHVD3 in serum, plasma

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of 1,25-Dihydroxyvitamin D3 (DHVD3). No significant cross-reactivity or interference between 1,25-Dihydroxyvitamin D3 (DHVD3) and analogues was observed.

Sensitivität: 9.92 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1,25-Dihydroxyvitamin D3 (DHVD3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1,25-Dihydroxyvitamin D3 (DHVD3) and unlabeled 1,25-Dihydroxyvitamin D3 (DHVD3) (Standards or samples) with the pre-coated antibody specific to 1,25-Dihydroxyvitamin D3 (DHVD3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1,25-Dihydroxyvitamin D3 (DHVD3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1,25-Dihydroxyvitamin D3 (DHVD3) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 1,25-Dihydroxyvitamin D3 (DHVD3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 1,25-Dihydroxyvitamin D3 (DHVD3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu 1,25 Dihydroxy Vitamin D3

Target: 1,25 Dihydroxy Vitamin D3 (DHVD3) (1,25-Dihydroxy Vitamin D3 (DHVD3))

Andere Bezeichnung: 1,25-Dihydroxyvitamin D3

Substanzklasse: Chemical

Hintergrund: Gene Aliases: DH-VD3, Calcitriol, Rocaltrol, Calcijex, 1,25-(OH) 2D3, 1,25-Dihydroxycholecalciferol, 1,25-Dihydroxy Vitamin D3