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Exosome Purification and Analysis Kit (Cell culture)

FACS Reaktivität: Human Fluorometric Cell Culture Cells
Produktnummer ABIN5633279
  • Reaktivität
    Human
    Nachweismethode
    Fluorometric
    Konjugat
    PE
    Applikation
    Flow Cytometry (FACS)
    Verwendungszweck
    Exosome purification and FACS analysis kit of exosomes derived from cell culture
    Marke
    ExoStep™
    Proben
    Cell Culture Cells
    Produktmerkmale
    The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
    Bestandteile
    CD63+ capture beads Anti-CD9 biotin Streptavidin-Phycoerythrin (PE) Assay Buffer 10X
    Benötigtes Material
    • Pre-enriched exosomes by ultra-centrifugation. • Magnetic Rack, MagneSphere(R) Mag. Sep. Stand 12-hole, 12x75mm (PROMEGA, Ref Z5343). • 12x75 mm Polystyrene Round Bottom Tubes (cytometer
  • tubes
  • ). • Sterile syringe filter with a 0.22 μm pore. (EMD Millipore, Ref SLGSV255F) • Syringe of adequate volume
  • Applikationshinweise
    Isolate and purify exosomes by FACS with reproducible results. You can calculate the concentration of protein easily and without other protein contaminations. It allows also immunophenotyping of the captured exosomes
    Kommentare

    Very reproducible and faster than WB or TME.3 hours for enrichement the sample+ Overnight Incubation+ 1 hour of protocol

    Probenmenge
    50 μL
    Testdauer
    4 h
    Aufbereitung der Reagenzien

    Reagents ready to use.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store in the dark at 2-8 °C
  • Hintergrund
    Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
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