Exosome Purification and Analysis Kit (Cell culture)

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Flow Cytometry (FACS)
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Verwendungszweck Exosome purification and FACS analysis kit of exosomes derived from cell culture
Marke ExoStep™
Proben Cell Culture Cells
Nachweismethode Fluorometric
Produktmerkmale The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
Bestandteile CD63+ capture beads Anti-CD9 biotin Streptavidin-Phycoerythrin (PE) Assay Buffer 10X
Benötigtes Material • Pre-enriched exosomes by ultra-centrifugation. • Magnetic Rack, MagneSphere(R) Mag. Sep. Stand 12-hole, 12x75mm (PROMEGA, Ref Z5343). • 12x75 mm Polystyrene Round Bottom Tubes (cytometer
  • tubes
  • ). • Sterile syringe filter with a 0.22 μm pore. (EMD Millipore, Ref SLGSV255F) • Syringe of adequate volume
    Hintergrund Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
    Applikationshinweise Isolate and purify exosomes by FACS with reproducible results. You can calculate the concentration of protein easily and without other protein contaminations. It allows also immunophenotyping of the captured exosomes

    Very reproducible and faster than WB or TME.3 hours for enrichement the sample+ Overnight Incubation+ 1 hour of protocol

    Probenmenge 50 μL
    Testdauer 4 h
    Aufbereitung der Reagenzien

    Reagents ready to use.

    Beschränkungen Nur für Forschungszwecke einsetzbar
    Buffer The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
    Konservierungsmittel Sodium azide
    Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung 4 °C
    Informationen zur Lagerung Store in the dark at 2-8 °C