Exosome Purification and Analysis Kit (Cell culture)

Produktnummer ABIN5633279

Kurzübersicht für Exosome Purification and Analysis Kit (Cell culture) (ABIN5633279)

Reaktivität: Human

Nachweismethode: Fluorometric

Applikation: Flow Cytometry (FACS)

Proben: Cell Culture Cells

Produktdetails

Konjugat: PE

Verwendungszweck: Exosome purification and FACS analysis kit of exosomes derived from cell culture

Marke: ExoStep™

Produktmerkmale: The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.

Bestandteile: CD63+ capture beads Anti-CD9 biotin Streptavidin-Phycoerythrin (PE) Assay Buffer 10X

Benötigtes Material: • Pre-enriched exosomes by ultra-centrifugation. • Magnetic Rack, MagneSphere(R) Mag. Sep. Stand 12-hole, 12x75mm (PROMEGA, Ref Z5343). • 12x75 mm Polystyrene Round Bottom Tubes (cytometer

tubes

). • Sterile syringe filter with a 0.22 μm pore. (EMD Millipore, Ref SLGSV255F) • Syringe of adequate volume

Anwendungsinformationen

Applikationshinweise: Isolate and purify exosomes by FACS with reproducible results. You can calculate the concentration of protein easily and without other protein contaminations. It allows also immunophenotyping of the captured exosomes

Kommentare:

Very reproducible and faster than WB or TME.3 hours for enrichement the sample+ Overnight Incubation+ 1 hour of protocol

Probenmenge: 50 μL

Testdauer: 4 h

Aufbereitung der Reagenzien:

Reagents ready to use.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store in the dark at 2-8 °C

Antigendetails

Hintergrund: Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.