Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

MYH1 ELISA Kit

Wide Range MYH1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Produktnummer ABIN5564702
  • Target Alle MYH1 ELISA Kits anzeigen
    MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1))
    Reaktivität
    • 3
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.31 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.31 ng/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität

    This assay has high sensitivity and excellent specificity for detection of Wide-range Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1).
    No significant cross-reactivity or interference between Wide-range Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) and analogues was observed.

    Sensitivität
    0.137 ng/mL
    Güteklasse
    Wide Range
    Bestandteile
    • Pre-coated, ready to use 96-well strip plate
    • Plate sealer for 96 wells
    • Standard Diluent
    • Assay Diluent A
    • Assay Diluent B
    • Stop Solution
    • Standard
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    Benötigtes Material
    • Microplate reader with 450 nm filter.
    • Precision single or multi-channel pipettes and disposable tips.
    • Eppendorf Tubes for diluting samples.
    • Deionized or distilled water.
    • Absorbent paper for blotting the microtiter plate.
    • Container for Wash Solution
    Featured
    Zu unserem meistverkauften MYH1 ELISA Kit
    Top Product
    Discover our top product MYH1 ELISA Kit
  • Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wide-range Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Aufbereitung der Reagenzien
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 20ng/mL. Prepare 7 tubes containing 0.5 mL Standard Diluent and produce a double dilution series by transferring 500 µL each. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for once pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. Prepare Substrate working Solution within 15 minutes before assay.
    6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    7. Contaminated water or container for reagent preparation will influence the detection result.
    Testdurchführung
    1. Prepare all reagents, samples and standards,
    2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
    4. Aspirate and wash 3 times,
    5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    6. Aspirate and wash 5 times,
    7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    8. Add 50μL Stop Solution. Read at 450nm immediately.
    Testpräzision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handhabung
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Lagerung
    4 °C
    Informationen zur Lagerung
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Haltbarkeit
    6 months
  • Target Alle MYH1 ELISA Kits anzeigen
    MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1))
    Abstract
    MYH1 Produkte
    Synonyme
    MYH1 ELISA Kit, MYH6 ELISA Kit, adult ELISA Kit, myosin ELISA Kit, A530084A17Rik ELISA Kit, IId ELISA Kit, IId/x ELISA Kit, MHC-2X/D ELISA Kit, MHC2X/D ELISA Kit, MYHC-IIX ELISA Kit, MdMs ELISA Kit, MyHC-IId/x ELISA Kit, MyHC-IIx/d ELISA Kit, Myhs-f ELISA Kit, Myhs-f2 ELISA Kit, Myhsf2 ELISA Kit, MYHC ELISA Kit, MYHSA1 ELISA Kit, MYHa ELISA Kit, MyHC-2X/D ELISA Kit, MyHC-2x ELISA Kit, MYHC-2X ELISA Kit, MYHC-2d ELISA Kit, myosin, heavy chain 1E, skeletal muscle ELISA Kit, myosin, heavy polypeptide 1, skeletal muscle, adult ELISA Kit, myosin heavy chain 1 ELISA Kit, myosin, heavy chain 1, skeletal muscle, adult ELISA Kit, myosin-8 ELISA Kit, myosin-1 ELISA Kit, myosin type I ELISA Kit, MYH1E ELISA Kit, Myh1 ELISA Kit, MYH1 ELISA Kit, LOC100303760 ELISA Kit, LOC100726730 ELISA Kit, myo1 ELISA Kit
    UniProt
    Q5SX40
Sie sind hier:
Kundenservice