Vasopressin ELISA Kit

Produktnummer ABIN5564691

Produktdetails Vasopressin ELISA Kit

Target: Vasopressin (AVP) (Arginine Vasopressin (AVP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 6.17 pg/mL - 500 pg/mL

Untere Nachweisgrenze: 6.17 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of High Sensitive Antidiuretic Hormone (ADH).
No significant cross-reactivity or interference between High Sensitive Antidiuretic Hormone (ADH) and analogues was observed.

Sensitivität: 2.65 pg/mL

Güteklasse: High Sensitivity

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard Diluent
• Assay Diluent A
• Assay Diluent B
• Stop Solution
• Standard
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Wash Buffer (30 x concentrate)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Probenmenge: 50 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Antidiuretic Hormone (ADH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Antidiuretic Hormone (ADH) and unlabeled High Sensitive Antidiuretic Hormone (ADH) (Standards or samples) with the pre-coated antibody specific to High Sensitive Antidiuretic Hormone (ADH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Antidiuretic Hormone (ADH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Antidiuretic Hormone (ADH) in the sample.

Testdurchführung:

1. Prepare all reagents, samples and standards,
   
2. Add 50μL standard or sample to each well.
   And then add 50μL prepared Detection Reagent A immediately.
   Shake and mix. Incubate 1 hour at 37 °C,
   
3. Aspirate and wash 3 times,
   
4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
   
5. Aspirate and wash 5 times,
   
6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
   
7. Add 50μL Stop Solution. Read at 450 nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Antidiuretic Hormone (ADH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Antidiuretic Hormone (ADH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Details zu Vasopressin

Target: Vasopressin (AVP) (Arginine Vasopressin (AVP))

Andere Bezeichnung: Antidiuretic Hormone (ADH) (AVP Produkte)

Synonyme: ADH ELISA Kit, ARVP ELISA Kit, AVP-NPII ELISA Kit, AVRP ELISA Kit, VP ELISA Kit, 2610201A18Rik ELISA Kit, Akr1a4 ELISA Kit, Vp ELISA Kit, Vsp ELISA Kit, DI ELISA Kit, Vas ELISA Kit, Adh ELISA Kit, Adh1a ELISA Kit, Adh1c ELISA Kit, adh ELISA Kit, arvp ELISA Kit, avrp ELISA Kit, avp-npii ELISA Kit, copeptin ELISA Kit, vasotocin ELISA Kit, arginine vasopressin ELISA Kit, aldo-keto reductase family 1, member A1 (aldehyde reductase) ELISA Kit, alcohol dehydrogenase 1 (class I) ELISA Kit, AVP ELISA Kit, Akr1a1 ELISA Kit, Avp ELISA Kit, Adh1 ELISA Kit, avp ELISA Kit

Pathways: cAMP Metabolic Process