Vitamin D-Binding Protein ELISA Kit

Produktnummer ABIN5526850

Das Maus Vitamin D-Binding Protein ELISA Kit (ABIN5526850) ist ein Colorimetric ELISA Kit zur Detektion von Maus Vitamin D-Binding Protein.

Kurzübersicht für Vitamin D-Binding Protein ELISA Kit (ABIN5526850)

Target: Vitamin D-Binding Protein (GC)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6.25 ng/mL - 400 ng/mL

Applikation: ELISA

Proben: Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate

Produktdetails Vitamin D-Binding Protein ELISA Kit

Untere Nachweisgrenze: 6.25 ng/mL

Verwendungszweck: Sandwich ELISA for quantitative detection of Mouse Vitamin D-binding protein concentrations.

Analytische Methode: Quantitative

Sensitivität: 2270 pg/mL

Bestandteile:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Benötigtes Material:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped and store at 4°C for 6 months, store at -20°C for one year.

Haltbarkeit: 6-12 months

Details zu Vitamin D-Binding Protein

Target: Vitamin D-Binding Protein (GC)

Andere Bezeichnung: Vitamin D-binding protein

Hintergrund: Alternative Names: Vitamin D-binding protein, DBP, VDB, Gc-globulin, Group-specific component

Gen-ID: 14473

UniProt: P21614

Pathways: Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process