Ribose 5-Phosphate Isomerase A (RPIA) ELISA Kit

Produktnummer ABIN5525552

Das Human Ribose 5-Phosphate Isomerase A ELISA Kit (ABIN5525552) ist ein Colorimetric ELISA Kit zur Detektion von Human Ribose 5-Phosphate Isomerase A.

Kurzübersicht für Ribose 5-Phosphate Isomerase A (RPIA) ELISA Kit (ABIN5525552)

Target: Ribose 5-Phosphate Isomerase A (RPIA)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 1.563 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails

Untere Nachweisgrenze: 1.563 ng/mL

Verwendungszweck: For quantitative detection of RPIA in serum, plasma, tissue homogenates.

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of RPIA. No significant cross-reactivity or interference between RPIA and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between RPIA and all the analogues, therefore, cross reaction may still exist.

Sensitivität: 0.938 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard
• Standard Dilution Buffer
• Biotin-labeled Antibody(Lyophilized)
• Purified water
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Competitive ELISA, Coated with Antigen

Probenmenge: 50 μL

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

• Bring all reagents and samples to room temperature for 20 minutes before use.
• Wash Buffer: If crystals have formed in the concentrate, you can warm it with 40 °C water bath Concentrated Wash Buffer into 750 mL Wash Buffer with deionized or distilled water. Put unused solution back at 2-8 °C.
• Standards:
  1. Add 1 mL Sample Dilution Buffer into one Standard tube (labeled as zero tube), keep the tube at room temperature for 10 minutes and mix them thoroughly. Note: If the standard tube concentration higher than the range of the kit,please dilute it and labeled as zero tube.
  2. Label 7 EP tubes with 1/2, 1/4, 1/8, 1/16, 1/32, 1/64 and blank respectively. Add 0.3 mLof the Sample Dilution Buffer into each tube. Add 0.3 mLof the above Standard solution (from zero tube) into 1st tube and mix them thoroughly. Transfer 0.3 mL from 1st tube to 2nd tube and mix them thoroughly. Transfer 0.3 mL from 2nd tube to 3rd tube and mix them thoroughly, and so on. Sample Dilution Buffer was used for the blank control. Note: It is best to use Standard Solutions within 2 hours.
• Preparation of Biotin-labeled Antibody Working Solution:
  Prepare it within 1 hour before experiment.
  1. Dissolve: Add 70ul purified water into tube and mix them thoroughly, after the biotin-labeled antibody is dissolved, please store it at 2-8°C.
  2. Calculate required total volume of the working solution: 0.05ml/well x quantity of wells. (Allow 0.1-0.2 mLmore than the total volume.)
  3. Dilute the Biotin-detection antibody with Antibody Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 µL Biotin-labeled antibody into 99 µL Antibody Dilution Buffer.)
• Preparation of HRP-Streptavidin Conjugate (SABC) Working Solution:
  Prepare it within 30 minutes before experiment.
  1. Calculate required total volume of the working solution: 0.1ml/well x quantity of wells. (Allow 0.1-0.2 mLmore than the total volume.)
  2. Dilute the SABC with SABC Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 µL of SABC into 99 µL of SABC Dilution Buffer.)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Haltbarkeit: 6 months

Antigendetails

Target: Ribose 5-Phosphate Isomerase A (RPIA)

Andere Bezeichnung: RPIA

Hintergrund: RPIA(Ribose-5-phosphate isomerase)/RPI/Phosphoriboisomerase

UniProt: P49247

Pathways: Cellular Glucan Metabolic Process, Ribonucleoside Biosynthetic Process