Retinoic Acid Receptor beta ELISA Kit

Produktnummer ABIN5521523

Das Maus Retinoic Acid Receptor beta ELISA Kit (ABIN5521523) ist ein Colorimetric ELISA Kit zur Detektion von Maus Retinoic Acid Receptor beta.

Kurzübersicht für Retinoic Acid Receptor beta ELISA Kit (ABIN5521523)

Target: Retinoic Acid Receptor beta (RARB) (Retinoic Acid Receptor, beta (RARB))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 46.875 pg/mL - 3000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Produktdetails Retinoic Acid Receptor beta ELISA Kit

Untere Nachweisgrenze: 46.875 pg/mL

Verwendungszweck: Mouse Rarβ (Retinoic acid receptor beta) ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with Rarβ , no obvious cross reaction with other analogues.

Sensitivität: 28.125 pg/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Sandwich ELISA, Double Antibody

Probenmenge: 100 μL

Testdauer: 4 h

Plattentyp: Pre-coated

Protokoll: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Details zu Retinoic Acid Receptor beta

Target: Retinoic Acid Receptor beta (RARB) (Retinoic Acid Receptor, beta (RARB))

Andere Bezeichnung: Rarbeta

Hintergrund: Rarβ, RARB, Retinoic acid receptor beta, RAR-beta, RAR-epsilon, HBV-activated protein, Nuclear receptor subfamily 1 group B member 2

UniProt: P22605

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway