12-Hydroxyeicosatetraenoic Acid ELISA Kit

Produktnummer ABIN5520240

Human 12-Hydroxyeicosatetraenoic Acid ELISA Kit, Colorimetric Assay für die Quantifizierung von Human 12-Hydroxyeicosatetraenoic Acid.

Kurzübersicht für 12-Hydroxyeicosatetraenoic Acid ELISA Kit (ABIN5520240)

Target: 12-Hydroxyeicosatetraenoic Acid

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.313 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails

Untere Nachweisgrenze: 0.313 ng/mL

Verwendungszweck: For quantitative detection of 12-HETE in serum, plasma, tissue homogenates.

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of 12-HETE. No significant cross- reactivity or interference between 12-HETE and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between 12-HETE and all the analogues, therefore, cross reaction may still exist.

Sensitivität: 0.188 ng/mL

Bestandteile: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Benötigtes Material: - Microplate reader (wavelength: 450nm)
- 37 °C incubator
- Automated plate washer
- Precision single and multi-channel pipette and disposable tips
- Clean tubes and Eppendorf tubes
- Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Competitive ELISA, Coated with Antigen

Probenmenge: 50 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Haltbarkeit: 6 months

Antigendetails

Target: 12-Hydroxyeicosatetraenoic Acid

Andere Bezeichnung: 12-HETE

Hintergrund: 12-HETE(12-Hydroxyeicosatetraenoic Acid)