SAA ELISA Kit

Produktnummer ABIN5519845

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Rind (Kuh) SAA.

Kurzübersicht für SAA ELISA Kit (ABIN5519845)

Target: SAA (Serum Amyloid A (SAA))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 9.375 ng/mL - 600 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails SAA ELISA Kit

Untere Nachweisgrenze: 9.375 ng/mL

Verwendungszweck: For quantitative detection of SAA in serum, plasma, tissue homogenates.

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of SAA. No significant cross-reactivity or interference between SAA and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between SAA and all the analogues, therefore, cross reaction may still exist.

Sensitivität: 5.625 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody (Concentrated)
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate
• Stop Solution
• Wash Buffer (25 x concentrate)
• Preprocess Agent A
• Preprocess Agent B
• Instruction manual

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Sandwich ELISA, Double Antibody

Probenmenge: 100 μL

Plattentyp: Pre-coated

Aufbereitung der Proben:

The user should estimate the concentration of target protein in the test sample, and select a proper dilution factor to make the diluted target protein concentration fall in the optimal detection range of the kit. Dilute the sample with the provided dilution buffer, and several trials may be necessary. The test sample must be well mixed with the dilution buffer. And also standard curves and sample should be making in pre-experiment. If samples with very high concentrations, dilute samples with PBS first and then dilute the samples with Sample Dilution. The matrix components in the sample will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing!
In order to reduce the influence of the substrate on the experiment, we suggest preprocess the sample.

• Serum/Plasma: Add 30ul of Preprocess Agent A to 60ul Serum/Plasma samples, mix well, incubate 10 minutes at room temperature. Then add 30ul of Preprocess Agent B, mix well. Note: The matrix components in the sample will affect the test results. After sample pretreatment, it is still necessary to dilute at least 1/2 with Sample Dilution Buffer before testing! The sample dilution caused by pretreatment should be considered in the calculation of the result.
• Cell Culture Supernatant: Add 20ul of Preprocess Agent A to 100ul samples, mix well, incubate 10 minutes at room temperature. Then add 20ul of Preprocess Agent B, mix well. At last, add 60ul Sample dilution buffer, mix well and test. Note: Sample is diluted for 2 fold dilution (1/2).The sample dilution caused by pretreatment should be considered in the calculation of the result.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Haltbarkeit: 6 months

Details zu SAA

Target: SAA (Serum Amyloid A (SAA))

Andere Bezeichnung: SAA

Hintergrund: SAA(Serum Amyloid A)

UniProt: P35541