Afamin ELISA Kit

Produktnummer ABIN5510657

Das Human Afamin ELISA Kit (ABIN5510657) ist ein Colorimetric ELISA Kit zur Detektion von Human Afamin. Das Kit wurde in 1 Publikation zitiert.

Kurzübersicht für Afamin ELISA Kit (ABIN5510657)

Target: Afamin (AFM)

Bindungsspezifität: AA 22-599

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Urine

Produktdetails Afamin ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich Human Afamin ELISA Kit to quantitate Human AFM in cell culture supernatants, serum and urine.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: L22-N599

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 4.9%, 5.1%, 5.8%
Inter-Assay Precision (CV%): 7.1%, 7.6%, 7.8%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für Afamin ELISA Kit (ABIN5510657)

Iori, Vinci, Murphy, Marescotti, Avogaro, Ahluwalia: "Glucose and fatty acid metabolism in a 3 tissue in-vitro model challenged with normo- and hyperglycaemia." in: PLoS ONE, Vol. 7, Issue 4, pp. e34704, (2012) (PubMed).

Details zu Afamin

Target: Afamin (AFM)

Andere Bezeichnung: AFM

Hintergrund:

Background: Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.

Gene Full Name: afamin

Gen-ID: 173

UniProt: P43652