MIA ELISA Kit

Produktnummer ABIN5068340

Das Human MIA ELISA Kit (ABIN5068340) ist ein Colorimetric ELISA Kit zur Detektion von Human MIA.

Kurzübersicht für MIA ELISA Kit (ABIN5068340)

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Plasma, Cell Culture Supernatant, Serum, Saliva

Produktdetails MIA ELISA Kit

Analytische Methode: Quantitative

Sensitivität: 8.46 pg/mL

Produktmerkmale: MIA, ELISA Kit (Human) (CD-RAP)

Bestandteile:

• Microplate, 1x96wells
• Conjugate, 1x21ml
• Standard, 1x10ng
• Assay Diluent, 1x11ml
• Callibrator Diluent, 1x21ml
• Wash buffer Concentrate, 1x21ml
• Color Reagent A, 1x12ml
• Color Reagent B, 1x12ml
• Stop Solution, 1x6ml
• Plate Sealers, 4x adhesive strips

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle:

• This employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human MIA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any human MIA present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for human MIA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human MIA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C/-20°C

Details zu MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Andere Bezeichnung: MIA

Hintergrund: MIA, also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is a secreted protein expressed in cartilaginous tissue during development and in the adult. MIA was identified based on the fact that it is highly expressed in malignant melanomas, but not in normal melanocytes. MIA has been shown to interact with fibronectin, and promotes invasion and metastasis of melanoma cells, perhaps by blocking integrin-fibronectin interactions.