MFGE8 ELISA Kit

Produktnummer ABIN5068339

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human MFGE8.

Kurzübersicht für MFGE8 ELISA Kit (ABIN5068339)

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Urine, Cell Culture Supernatant, Plasma (EDTA), Serum, Plasma (heparin), Saliva, Milk

Produktdetails MFGE8 ELISA Kit

Analytische Methode: Quantitative

Sensitivität: 13.4 pg/mL

Produktmerkmale: MFG-E8, Human, ELISA Kit (BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, SED1)

Bestandteile:

• Mfg-e8 Microplate, 1x96wells
• Mfg-e8 Standard, lyophilized, 1x40ng
• Mfg-e8 Conjugate, 1x21ml
• Assay Diluent r, 1x11ml
• Calibrator Diluent r, 1x21ml
• Wash Buffer Concentrate, 25X concentrated, 1x21ml
• Color Reagent A, 1x12ml
• Color Reagent B, 1x12ml
• Stop Solution, 1x6ml
• Plate Sealers, 4x adhesive strips

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle:

• This employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human MFG-E8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any human MFG-E8 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for human MFG-E8 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human MFG-E8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C/-20°C

Details zu MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Andere Bezeichnung: MFG-E8

Hintergrund: MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.

Pathways: SARS-CoV-2 Protein Interaktom