Serotonin ELISA Kit

Produktnummer ABIN5068229

Dieses Chemical Serotonin ELISA-Kit ist ein Fluorometric, Colorimetric ELISA-Kit, das dafür entwickelt wurde, Chemical Serotonin zu quantifizieren.

Kurzübersicht für Serotonin ELISA Kit (ABIN5068229)

Target: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Reaktivität: Chemical

Nachweismethode: Fluorometric, Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Proben: Cell Culture Lysate, Plasma, Serum

Produktdetails Serotonin ELISA Kit

Analytische Methode: Quantitative

Sensitivität: 0.08-19.8 ng/mL

Produktmerkmale: Serotonin, Human (5-Hydroxytryptamine, 5HT, 5-HT, 5-HT5A, 5-HT-5A) ELISA Kit

Bestandteile:

• Microtiter Plate, 1x96 wells
• Serotonin Pab 1x8ml
• Serotonin (Biotin) Pab, 1x8ml
• Streptavidin (AP), 100x, 1x300ul
• Standard, 0ng/ml 1x1ml
• Standard, 0.08ng/ml 1x1ml
• Standard, 0.24ng/ml 1x1ml
• Standard, 0.73ng/ml 1x1ml
• Standard, 2.2ng/ml 1x1ml
• 0: Standard, 6.6ng/ml 1x1ml
• 1: Standard, 19.8ng/ml 1x1ml
• 2: Control 1, ng/ml, 1x500ul
• 3: Control 2, ng/ml, 1x500ul
• 4: Acylation Reagent, 1x3ml
• 5: Assay Buffer, 10x, 1x50ml
• 6: Wash Buffer, 20x, 2x50ml
• 7: Substrate (PNPP), 2x13ml
• 8: Stop Solution (1N NaOH), 1x15ml

Anwendungsinformationen

Protokoll: Principle:

• The sample preparation (derivatization of serotonin to N-acylserotonin) is part of the sample dilution and is achieved by incubation of the respective sample with the Acylation Reagent.
• The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C

Details zu Serotonin

Target: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Andere Bezeichnung: Serotonin

Substanzklasse: Chemical