IgG3 ELISA Kit

Produktnummer ABIN5068222

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus IgG3.

Kurzübersicht für IgG3 ELISA Kit (ABIN5068222)

Target: IgG3

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Ascitic Fluid, Colostrum, Milk, Mucosa, Saliva, Tears

Produktdetails IgG3 ELISA Kit

Sensitivität: 25 ng/mL

Produktmerkmale: IgG3, Mouse, ELISA Kit

Bestandteile:

• Anti-Mouse IgG3 Microwell Strip Plate: 12X8-well strips
• Mouse IgG3 Control: 1X0.65ml
• Mouse IgG3 Standard (50ng/ml): 1X0.65ml
• Mouse IgG3 Standard (100ng/ml): 1X0.65ml
• Mouse IgG3 Standard (400ng/ml): 1X0.65ml
• Mouse IgG3 Standard (400ng/ml): 1X0.65ml
• Mouse IgG3 Standard (1000ng/ml): 1X0.65ml
• Anti-Mouse IgG3 HRP Conjugate (100X): 1X0.15ml
• Sample Diluent Concentrate (20X): 1X10ml
• Wash Solution Concentrate (100X): 1X10ml
• TMB Substrate: 1X12ml
• Stop Solution: 1X12ml

Anwendungsinformationen

Protokoll: Principle:

• The Mouse IgG3 ELISA kit is based on the binding of mouse IgG3 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgG3 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgG3 in samples and control is calculated from a curve of standards containing known concentrations of IgG3.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C

Details zu IgG3

Target: IgG3

Substanzklasse: Antibody

Hintergrund: Immunoassays using heavy-chain specific antibodies provide for selective, sensitive quantification of mouse immunoglobulins IgG (and subclasses), IgA and IgM, as found circulating in blood or as present in other body fluids, including saliva, milk/colostrum, ascites, tears and mucosa of linings of the gut, respiratory or urigenital tracts.