Triiodothyronine T3 ELISA Kit

Produktnummer ABIN5068110

Human Triiodothyronine T3 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Triiodothyronine T3.

Kurzübersicht für Triiodothyronine T3 ELISA Kit (ABIN5068110)

Target: Triiodothyronine T3 (T3)

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum

Produktdetails Triiodothyronine T3 ELISA Kit

Verwendungszweck: Triiodothyronine, Free, Human, ELISA Kit (fT3, Free T3) is based on competitive binding of human free thyroxine from serum samples and enzyme-labeled T3 to T3-specific antibodies immobilized on microtiter well plates. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (blue color) is inversely proportional to the amount of T3 present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow). Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of free T3 in samples and control is read off the standard curve.

Analytische Methode: Quantitative

Sensitivität: 0.3 pg/mL

Produktmerkmale: Triiodothyronine, Free, Human, ELISA Kit (fT3, Free T3)

Bestandteile:

• Microstrip Plate, 1x96wells
• Assay Buffer, 1x15ml
• Sample Diluent/Standard 0pg/ml 1x10ml
• Standard 2pg/ml 1x500ul
• Standard 4pg/ml 1x500ul
• Standard 8pg/ml 1x500ul
• Standard 16pg/ml 1x500ul
• Standard 40pg/ml 1x500ul
• Low Control (3..5pg/ml), 1x500ul
• 0: High Control (6..5pg/m). 1x500ul
• 1: Ab (HRP), 50X, 1x300ul
• 2: Wash Buffer, 10X, 1x50ml
• 3: TMB Solution, 1x16ml
• 4: Stop Solution (1M sulfuric acid), 1x6ml

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C/-20°C

Details zu Triiodothyronine T3

Target: Triiodothyronine T3 (T3)

Andere Bezeichnung: Triiodothyronine

Substanzklasse: Amino Acid