Kynurenine ELISA Kit

Produktnummer ABIN5067984

Chemical Kynurenine ELISA Kit, Colorimetric Assay für die Quantifizierung von Chemical Kynurenine.

Kurzübersicht für Kynurenine ELISA Kit (ABIN5067984)

Target: Kynurenine

Reaktivität: Chemical

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails Kynurenine ELISA Kit

Verwendungszweck: The Kynurenine (KYN) ELISA Kit is a quantitative competitive assay for the detection of KYN in human serum, plasma, tissue homogenates and other biological fluids.

Analytische Methode: Quantitative

Sensitivität: > 4.69 pM/mL

Produktmerkmale: Kynurenine (KYN) ELISA Kit (Human)

Bestandteile:

• *356970A: Microtiter Strips, 1x96 wells (8x12 wells).
• *356970B: Standard, 2x1 vial
• 356970C: Sample/Standard Dilution Buffer, 1x20ml
• 356970D: Antibody (Biotin) (Concentrated), 1x60ul
• 356970E: Antibody Dilution Buffer, 1x10ml
• 356970F: Streptavidin (HRP) (SABC), 1x120ul
• 356970G: SABC Dilution Buffer, 1x10ml
• 356970H: TMB Substrate, 1x10ml
• 356970J: Stop Solution, 1x10ml
• 356970K: Wash Buffer, 25X, 1x30ml

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle:

• The microtiter plate provided in this kit has been pre-coated with KYN. KYN in the standard or sample competes with a fixed amount of plate-coated KYN for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of KYN in the samples is determined by comparing the absorbance of the samples to the standard curve.

Testpräzision: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C/-20°C

Details zu Kynurenine

Target: Kynurenine

Andere Bezeichnung: Kynurenine (KYN)

Substanzklasse: Chemical