IgG1 ELISA Kit

Produktnummer ABIN5067980

Dieses Maus IgG1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus IgG1 zu quantifizieren.

Kurzübersicht für IgG1 ELISA Kit (ABIN5067980)

Target: IgG1

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Culture Cells, Serum, Saliva

Produktdetails IgG1 ELISA Kit

Verwendungszweck: The IgG1 Murine BioAssay ELISA Kit is an in vitro immunoassay for the quantification of IgG1 circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.

Analytische Methode: Quantitative

Sensitivität: 5 ng/mL

Produktmerkmale: IgG1, Mouse, ELISA Kit

Bestandteile:

• Microtiter Plate: 1x96 wells
• Positive Control (ng/ml): 1x650ul
• Standard 5ng/ml, 1x650ul
• Standard 10ng/ml, 1x650ul
• Standard 20ng/ml, 1x650ul
• Standard 40ng/ml, 1x650ul
• Standard 100ng/ml, 1x650ul
• IgG1 (HRP), 100X, 1x150ul
• Sample Diluent, 20X, 1x10ml
• Wash Solution, 100X, 1x10ml
• TMB Substrate, 1x12ml
• Stop Solution, 1x12ml

Anwendungsinformationen

Protokoll: Principle:

• The Mouse IgG1 ELISA kit is based on the binding of mouse IgG1 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgG1 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgG1 in samples and control is calculated from a curve of standards containing known concentrations of IgG1.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C

Details zu IgG1

Target: IgG1

Substanzklasse: Antibody