IgA ELISA Kit
Kurzübersicht für IgA ELISA Kit (ABIN5067962)
Target
Alle IgA ELISA Kits anzeigenReaktivität
Nachweismethode
Applikation
Proben
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Verwendungszweck
- The Human IgA ELISA Kit is an in vitro immunoassay for research use in the quantification of human IgA circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of human cells.
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Analytische Methode
- Quantitative
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Sensitivität
- 20 ng/mL
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Produktmerkmale
- IgA, Human, ELISA Kit
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Bestandteile
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- Coated Microwell Plate: 12x8-well strips
- Human IgA Positive Control (ng/ml), 1x 650ul
- Human IgA Standard 50ng/ml, 1x 650ul
- Human IgA Standard 100ng/ml, 1x 650ul
- Human IgA Standard 250ng/ml, 1x 650ul
- Human IgA Standard 500ng/ml, 1x 650ul
- Human IgA Standard 1000ng/ml, 1x 650ul
- IgA (HRP) Conjugate, 100X, 1x 150ul
- Sample Diluent, 20X, 1x10ml
- Wash Solution, 100X, 1x10ml
- TMB Substrate: 1x12ml
- Stop Solution: 1x12ml
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IgA ELISA Kit
Reaktivität: Rind (Kuh) Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgA ELISA KitReaktivität: Maus Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
IgA ELISA KitReaktivität: Human Colorimetric Sandwich ELISA 0.14 ng/mL - 100 ng/mL Plasma, Saliva, Serum
IgA ELISA KitReaktivität: Schaf Colorimetric Competition ELISA 3.75 μg/mL - 60 μg/mL Plasma, Serum
IgA ELISA KitReaktivität: Maus Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
IgA ELISA KitReaktivität: Huhn Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Plasma, Serum
IgA ELISA KitReaktivität: Schwein Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
IgA ELISA KitReaktivität: Huhn Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
IgA ELISA KitReaktivität: Maus Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgA ELISA KitReaktivität: Human Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Urine
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Protokoll
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Principle:
- The Human IgA ELISA kit is based on the binding of human IgA in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgA present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgA in samples and control is calculated from a curve of standards containing known concentrations of IgA.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- 4°C
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- IgA
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Substanzklasse
- Antibody
Target Alle IgA ELISA Kits anzeigen
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