GFAP ELISA Kit

Produktnummer ABIN5067956

Dieses Human GFAP ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human GFAP in Cell Culture Lysate, Plasma und Serum zu quantifizieren.

Kurzübersicht für GFAP ELISA Kit (ABIN5067956)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Culture Lysate, Plasma, Serum

Produktdetails GFAP ELISA Kit

Verwendungszweck: The Human GFAP ELISA is a biotin-labeled-antibody-based sandwich enzyme immunoassay for the quantitative measurement of human GFAP in serum, CSF, plasma and tissue culture medium. It is intended for in vitro and research use only.

Analytische Methode: Quantitative

Sensitivität: 0.045 ng/mL

Produktmerkmale: GFAP, Human, ELISA Kit (Glial Fibrillary Acidic Protein)

Bestandteile:

• Microtiter Strips, coated with capture polyclonal antibody, sealed, 1x96 wells
• * Human GFAP Master Calibrator, 1x1 vial
• * Quality Control: High, 6..8ng/ml, 1x1 vial
• * Quality Control: Low, 1..6ng/ml, 1x1 vial
• Pab (Biotin), 1x13ml
• Streptavidin (HRP), 1x13ml
• Standard Diluent, 1x9ml
• Dilution Buffer, 1x13ml
• Wash Solution, 10X, 1x100ml
• TMB Substrate Solution, 1x13ml
• Stop Solution, O4, 1x13ml

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle:

• In Human GFAP ELISA calibrators or samples are incubated with a rabbit polyclonal anti-human GFAP antibody coated in microtiter wells. After two hours of incubation and a washing, biotin-labeled monoclonal anti-human GFAP antibody is added and incubated with captured GFAP. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour of incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by the addition of acidic solution. The absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of GFAP. A standard curve is constructed by plotting absorbance values versus GFAP concentrations of calibrators, and concentrations of unknown samples are determined using this standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C/-20°C

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP