Pepsin ELISA Kit
Kurzübersicht für Pepsin ELISA Kit (ABIN5067908)
Target
Alle Pepsin ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
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Verwendungszweck
- PP (Pepsin) ELISA Kit is for the quantitative detection of Pepsin in porcine serum, plasma, tissue homogenates and other biological fluids.
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Analytische Methode
- Quantitative
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Sensitivität
- 0.938 ng/mL
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Produktmerkmale
- PP (Pepsin) ELISA Kit (Porcine)
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Bestandteile
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- *358254A: Microtiter Strips, 1x96 wells (8x12 wells).
- *358254B: Standard, 2x1 vial
- 358254C: Sample/Standard Dilution Buffer, 1x20ml
- 358254D: Antibody (Biotin) (Concentrated), 1x120ul
- 358254E: Antibody Dilution Buffer, 1x10ml
- 358254F: Streptavidin (HRP) (SABC), 1x120ul
- 358254G: SABC Dilution Buffer, 1x10ml
- 358254H: TMB Substrate, 1x10ml
- 358254J: Stop Solution, 1x10ml
- 358254K: Wash Buffer, 25X, 1x30ml
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Pepsin ELISA Kit
Reaktivität: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Pepsin ELISA KitReaktivität: Human Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitReaktivität: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitReaktivität: Maus Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitReaktivität: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Pepsin ELISA KitReaktivität: Ratte Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitReaktivität: Maus Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitInstant Reaktivität: Human Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitSmall Sample Reaktivität: Maus Colorimetric Sandwich ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitReaktivität: Schwein Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
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Plattentyp
- Pre-coated
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Protokoll
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Principle:
- This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to Pepsin. Standards and samples are added to the appropriate wells, then incubated. Aspirate. Do not wash the plate! The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain Pepsin, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pepsin in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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Testpräzision
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Precision:
- Intra-Assay CV: <8%
- Inter-Assay CV: <10%
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- 4°C/-20°C
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- Pepsin
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Andere Bezeichnung
- PP (Pepsin)
Target Alle Pepsin ELISA Kits anzeigen
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