Transferrin Receptor ELISA Kit

Produktnummer ABIN5067899

Produktdetails Transferrin Receptor ELISA Kit

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human sTfR ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum, plasma and tissue culture medium.

Proben: Cell Culture Lysate, Plasma, Serum

Analytische Methode: Quantitative

Sensitivität: 2 ng/mL

Produktmerkmale: Transferrin Receptor, soluble, Human, ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)

Bestandteile:

• Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab
• Mab (HRP), 1x13ml
• Standard, 2ug/ml, 1x100ul
• Standard, 1ug/ml, 1x100ul
• Standard, 0.5ug/ml, 1x.100ul
• Standard, 0.2ug/ml, 1x100ul
• Standard, 0.1ug/ml, 1x100ul
• Standard, 0.05ug/ml, 1x100ul
• Quality Control, High, 50X, (5..3ug /ml), 1x50ul
• Quality Control, Low, 50X, (1..0ug/ml), 1x50ul
• Dilution Buffer, 2x13ml
• Wash Solution, 10X, 1x100ml
• Substrate Solution (TMB), 1x13ml
• Stop Solution (0.2M O4), 1x13ml

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle:

• Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody'sTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C

Details zu Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Andere Bezeichnung: Transferrin Receptor (TFRC Produkte)

Synonyme: Trfr ELISA Kit, LOC100219280 ELISA Kit, tfr ELISA Kit, cd71 ELISA Kit, tfr1 ELISA Kit, trfr ELISA Kit, CD71 ELISA Kit, T9 ELISA Kit, TFR ELISA Kit, TFR1 ELISA Kit, TR ELISA Kit, TRFR ELISA Kit, p90 ELISA Kit, 2610028K12Rik ELISA Kit, AI195355 ELISA Kit, AI426448 ELISA Kit, AU015758 ELISA Kit, E430033M20Rik ELISA Kit, Mtvr-1 ELISA Kit, Mtvr1 ELISA Kit, transferrin receptor ELISA Kit, TFRC ELISA Kit, Tfrc ELISA Kit, tfrc ELISA Kit

Pathways: Transition Metal Ion Homeostasis