Transferrin Receptor ELISA Kit

Produktnummer ABIN5067899

Dieses Human Transferrin Receptor ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human Transferrin Receptor zu quantifizieren.

Kurzübersicht für Transferrin Receptor ELISA Kit (ABIN5067899)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Culture Lysate, Plasma, Serum

Produktdetails Transferrin Receptor ELISA Kit

Verwendungszweck: Human sTfR ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum, plasma and tissue culture medium.

Analytische Methode: Quantitative

Sensitivität: 2 ng/mL

Produktmerkmale: Transferrin Receptor, soluble, Human, ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)

Bestandteile:

• Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab
• Mab (HRP), 1x13ml
• Standard, 2ug/ml, 1x100ul
• Standard, 1ug/ml, 1x100ul
• Standard, 0.5ug/ml, 1x.100ul
• Standard, 0.2ug/ml, 1x100ul
• Standard, 0.1ug/ml, 1x100ul
• Standard, 0.05ug/ml, 1x100ul
• Quality Control, High, 50X, (5..3ug /ml), 1x50ul
• Quality Control, Low, 50X, (1..0ug/ml), 1x50ul
• Dilution Buffer, 2x13ml
• Wash Solution, 10X, 1x100ml
• Substrate Solution (TMB), 1x13ml
• Stop Solution (0.2M O4), 1x13ml

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle:

• Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody'sTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C

Details zu Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Andere Bezeichnung: Transferrin Receptor

Pathways: Transition Metal Ion Homeostasis