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Resistin ELISA Kit

RETN Reaktivität: Human Colorimetric Sandwich ELISA Plasma, Serum, Tissue Culture Medium
Produktnummer ABIN5067898
  • Target Alle Resistin (RETN) ELISA Kits anzeigen
    Resistin (RETN)
    Reaktivität
    • 8
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.
    Proben
    Plasma, Serum, Tissue Culture Medium
    Analytische Methode
    Quantitative
    Sensitivität
    0.012 ng/mL
    Produktmerkmale
    Resistin, Human, ELISA Kit
    Bestandteile
    • Microtiter Plate, 1x96wells
    • Standard, 1x1vial
    • High Control, 1x1vial
    • Low Control, 1x1vial
    • Pab (Biotin), 1x13ml
    • Streptavidin (HRP), 1x13ml
    • Dilution Buffer, 1x10ml
    • Wash Solution (10X), 1x100ml
    • Substrate Solution (TMB), 1x13ml
    • Stop Solution (O4), 1x13ml
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  • Protokoll
    Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Informationen zur Lagerung
    4°C
  • Target Alle Resistin (RETN) ELISA Kits anzeigen
    Resistin (RETN)
    Andere Bezeichnung
    Resistin (RETN Produkte)
    Synonyme
    RETN ELISA Kit, ADSF ELISA Kit, FIZZ3 ELISA Kit, RETN1 ELISA Kit, RSTN ELISA Kit, XCP1 ELISA Kit, Fizz3 ELISA Kit, Rstn ELISA Kit, Xcp4 ELISA Kit, resistin ELISA Kit, RETN ELISA Kit, Retn ELISA Kit
    Pathways
    Feeding Behaviour, Smooth Muscle Cell Migration
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