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Resistin ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Resistin.

Produktnummer ABIN5067898

Datenblatt (PDF)

Kurzübersicht für Resistin ELISA Kit (ABIN5067898)

Target

Alle Resistin (RETN) ELISA Kits anzeigen

Resistin (RETN)

Reaktivität

Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Plasma, Serum, Tissue Culture Medium

Discover our top product RETN ELISA Kit

Verwendungszweck

Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.

Analytische Methode

Quantitative

Sensitivität

0.012 ng/mL

Produktmerkmale

Resistin, Human, ELISA Kit

Bestandteile
- Microtiter Plate, 1x96wells
- Standard, 1x1vial
- High Control, 1x1vial
- Low Control, 1x1vial
- Pab (Biotin), 1x13ml
- Streptavidin (HRP), 1x13ml
- Dilution Buffer, 1x10ml
- Wash Solution (10X), 1x100ml
- Substrate Solution (TMB), 1x13ml
- Stop Solution (O4), 1x13ml
Resistin (RETN) ELISA Kit
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RETN Reaktivität: Schaf Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate

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RETN Reaktivität: Kaninchen Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

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RETN Reaktivität: Schaf Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum

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RETN Reaktivität: Human AA 19-108 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Protokoll

Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Lagerung

4 °C

Informationen zur Lagerung

4°C
Target Alle Resistin (RETN) ELISA Kits anzeigen

Resistin (RETN)

Andere Bezeichnung

Resistin

Pathways

Feeding Behaviour, Smooth Muscle Cell Migration