Thyroglobulin CLIA Kit

Produktnummer ABIN504791

Dieses Chemiluminescent ELISA kit dient der quantitativen Messung von Human Thyroglobulin.

Kurzübersicht für Thyroglobulin CLIA Kit (ABIN504791)

Target: Thyroglobulin (TG)

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Applikation: ELISA

Produktdetails Thyroglobulin CLIA Kit

Verwendungszweck: Human thyroglobulin (Tg) is a large glycoprotein (660 kD) that is stored in the follicular colloid of the thyroid gland. It functions as a prohormone in the intra thyroid synthesis of primary thyroid hormones triiodothyronine (T3) and thyroxine (T4). Tg is elevated in thyroid follicular and papillary carcinoma, thyroid adenoma, subacute thyroiditis, Hashimotos thyroiditis and Graves Disease. Tg levels are found to be normal in patients with medullary thyroid carcinoma. Serial measurements of Tg are most useful in detecting recurrence of differentiated thyroid carcinoma following surgical resection or radioactive iodine ablation. Tg determination is used as an adjunct to iodine scanning but not as a replacement for it. Assessment of Tg levels aids in management of infants with congenital hypothyroidism. Tg determination has been done with various methods using direct competitive binding RIA and double antibody sandwich IRMA or Elisa, of which the latter is more useful. All these methods suffer from interference by endogenous autoantibodies to Tg. It is useful to determine the effect of autoantibodies before screening such patients for levels of Tg.We provide Tg autoantibody Elisa to rule out such interference.

Analytische Methode: Quantitative

Produktmerkmale: Monobinds Thyroglobulin Acculite Chemiluminescence test is intended to be used for the quantitative determination of thyroglobulin levels in human serum. The test is for in vitro diagnostic use only.

Anwendungsinformationen

Applikationshinweise: All products that contain human serum have been found to be non-reactive for Hepatitis B Surface Antigen, HIV 1&2 and HCV Antibodies by FDA required tests. Since no known test can offer complete assurance that infectious agents are absent, all human serum products should be handled as potentially hazardous and capable of transmitting disease. Good laboratory procedures for handling blood products can be found in the Center for Disease Control / National Institute of Health, "Biosafety in Microbiological and Biomedical Laboratories," 2nd Edition, 1988, HHS Publication No. (CDC) 88-8395.

Probenmenge: 50 μL

Plattentyp: Pre-coated

Protokoll:

Specimien Collection and Preparation:

The specimens shall be blood serum in type and the usual precautions in the collection of venipuncture samples should be observed. For accurate comparison to established normal values, a fasting morning serum sample should be obtained. The blood should be collected in a plain red-top venipuncture tube without additives or gel barrier. Allow the blood to clot. Centrifuge the specimen to separate the serum from the cells. Samples may be refrigerated at 2_x001E_8(C for a maximum period of five (5) days. If the specimen(s) cannot be assayed within this time, the sample(s) may be stored at temperatures of _x001E_20(C for up to 30 days. Avoid repetitive freezing and thawing. When assayed in duplicate, 0.100ml of the specimen is required.

Reagent Preparation:

1. Wash Buffer Dilute contents of Wash Concentrate to 1000ml with distilled or deionized water in a suitable storage container. Store diluted buffer at room temperature 20-27(C. 2. Working Signal Reagent Solution - Store at 2 - 8(C. Determine the amount of reagent needed and prepare by mixing equal portions of Signal Reagent A and Signal Reagent B in a clean container. For example, add 1 ml of A and 1ml of B per two (2) eight well strips (A slight excess of solution is made). Discard the unused portion if not used within 36 hours after mixing. If complete utilization of the reagents is anticipated, within the above time constraint, pour the contents of Signal Reagent B into Signal Reagent A and label accordingly.

Test Procedure:

Before proceeding with the assay, bring all reagents, serum references and controls to room temperature (20 - 27(C). 1. Format the microplates wells for each calibrator, control and patient sample to be assayed in duplicate. Replace any unused white microwell strips back into the aluminum bag, seal and store at 2-8(C. 2. Pipette 0.050 ml (50l) of the appropriate calibrators, controls and samples into the assigned wells. 3. Add 0.100 ml (100l) of the biotin labeled monoclonal antibody to each well. It is very important to dispense all reagents close to the bottom of the microwell. 4. Swirl the microplate gently for 20-30 seconds to mix. Cover with a plastic wrap. 5. Incubate for 12-18 hours at room temperature (overnight). 6. Discard the contents of the microplate by decantation or aspiration. If decanting, tap and blot the plate dry with absorbent paper. 7. Add 350l of wash buffer (see Reagent Preparation Section), decant (tap and blot) or aspirate. Repeat four (4) additional times for a total of five (5) washes. An automatic or manual plate washer can be used. Follow the manufacturers instruction for proper usage. If a squeeze bottle is used, fill each well to the top by squeezing the container. Avoiding air bubbles. Decant the wash and repeat four (4) additional times. Add 0.100 ml (100l) of Tg Tracer solution to all wells 9. Swirl the microplate gently for 20-30 seconds to mix. Cover with a plastic wrap. 10. Incubate at room temperature for 120 minutes. 11. Discard the contents of the microplate by decantation or aspiration. If decanting, blot the plate dry with absorbent paper. 12. Add 350l of wash buffer (see Reagent Preparation Section), decant (tap and blot) or aspirate. Repeat four (4) additional times for a total of five (5) washes. An automatic or manual plate washer can be used. Follow the manufacturers instruction for proper usage. If a squeeze bottle is employed, fill each well by depressing the container (avoiding air bubbles) to dispense the wash. Decant the wash and repeat four (4) additional times. 13. Add 0.100 ml (100l) of working signal reagent to all wells (see Reagent Preparation Section). Always add reagents in the same order to minimize reaction time. 14. Incubate at room temperature for five (5) minutes in the dark. 15. Read the Relative Light Units (RLU) in each well for 0.5 1.0 seconds. The results should be read within thirty (30) minutes. ALTERNATIVE SHORT PROCEDURE: This procedure can be used with the help of a laboratory hematology shaker 1. Format the microplates wells for each calibrator, control and patient sample to be assayed in duplicate. Replace any unused microwell strips back into the aluminum bag, seal and store at 2-8(C. 2. Pipette 0.050 ml (50l) of the appropriate calibrators, controls and samples into the assigned wells. 3. Add 0.100 ml (100l) of the biotin labeled monoclonal antibody to each well. It is very important to dispense all reagents close to the bottom of the microwell. 4. Incubate at room temperature for 2 hours while shaking constantly on a hematology shaker at 150 RPM. 5. Follow steps 6-15 as described in the Test Procedure above.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Antigendetails

Target: Thyroglobulin (TG)

Hintergrund: Human thyroglobulin (Tg) is a large glycoprotein (660 kD) that is stored in the follicular colloid of the thyroid gland. It functions as a prohormone in the intra thyroid synthesis of primary thyroid hormones triiodothyronine (T3) and thyroxine (T4). Tg is elevated in thyroid follicular and papillary carcinoma, thyroid adenoma, subacute thyroiditis, Hashimotos thyroiditis and Graves Disease. Tg levels are found to be normal in patients with medullary thyroid carcinoma. Serial measurements of Tg are most useful in detecting recurrence of differentiated thyroid carcinoma following surgical resection or radioactive iodine ablation. Tg determination is used as an adjunct to iodine scanning but not as a replacement for it. Assessment of Tg levels aids in management of infants with congenital hypothyroidism. Tg determination has been done with various methods using direct competitive binding RIA and double antibody sandwich IRMA or Elisa, of which the latter is more useful. All these methods suffer from interference by endogenous autoantibodies to Tg. It is useful to determine the effect of autoantibodies before screening such patients for levels of Tg. Monobind provides Tg autoantibody Elisa to rule out such interference. (Please see Monobinds Anti-Tg Acculite Cat# 1075-300).

Pathways: Thyroid Hormone Synthesis