alpha Fetoprotein ELISA Kit

Produktnummer ABIN5026955

Das Human alpha Fetoprotein ELISA Kit (ABIN5026955) ist ein Colorimetric ELISA Kit zur Detektion von Human alpha Fetoprotein.

Kurzübersicht für alpha Fetoprotein ELISA Kit (ABIN5026955)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.313-20 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails alpha Fetoprotein ELISA Kit

Untere Nachweisgrenze: 0.313 ng/mL

Verwendungszweck: This assay employs an antibody specific for Anti-human AFP coated on a 96-well plate.

Analytische Methode: Quantitative

Sensitivität: 0.188 ng/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human AFP concentrations in serum, plasma. Detection Range: 0.313 - 20 ng/mL Sensitivity: < 0.188 ng/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human AFP coated on a 96-well plate. Standards and samples are pipetted into the wells and AFP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human AFP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AFP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Please refer to the Manual

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Andere Bezeichnung: AFP (Alpha Fetoprotein)

Pathways: C21-Steroid Hormone Metabolic Process