TIMP2 ELISA Kit

Produktnummer ABIN5026950

Human TIMP2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human TIMP2.

Kurzübersicht für TIMP2 ELISA Kit (ABIN5026950)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156-10 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails TIMP2 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: This assay employs an antibody specific for Anti-human TIMP-2 coated on a 96-well plate.

Analytische Methode: Quantitative

Sensitivität: 0.094 ng/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human TIMP-2 concentrations in serum, plasma. Detection Range: 0.156 - 10 ng/mL Sensitivity: < 0.094 ng/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human TIMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Please refer to the Manual

Details zu TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Andere Bezeichnung: TIMP-2 (Metalloproteinase Inhibitor-2)

Pathways: cAMP Metabolic Process