ALOX15 ELISA Kit

Produktnummer ABIN5026944

Human ALOX15 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human ALOX15.

Kurzübersicht für ALOX15 ELISA Kit (ABIN5026944)

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails ALOX15 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human 15-LO concentrations in serum, plasma and other biological fluids. This kit is for Research Use Only. Not for use in diagnostic/therapeutics procedures.

Analytische Methode: Quantitative

Sensitivität: < 37.5 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human 15-LO coated on a 96-well plate. Standards and samples are pipetted into the wells and 15-LO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human 15-LO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of 15-LO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Andere Bezeichnung: 15-LO (Arachidonate 15-Lipoxygenase)

Pathways: Regulation of Actin Filament Polymerization