IL-33 ELISA Kit

Produktnummer ABIN5026939

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human IL-33.

Kurzübersicht für IL-33 ELISA Kit (ABIN5026939)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-33 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human IL-33 concentrations in serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Sensitivität: < 9.4 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-humanIL-33 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-33 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-33 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-33 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Andere Bezeichnung: IL-33 (Interleukin-33)

Pathways: Production of Molecular Mediator of Immune Response