LDL ELISA Kit

Produktnummer ABIN4993796

Das Human LDL ELISA Kit (ABIN4993796) ist ein Colorimetric ELISA Kit zur Detektion von Human LDL.

Kurzübersicht für LDL ELISA Kit (ABIN4993796)

Target: LDL (Low Density Lipoprotein (LDL))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6.32 pg/mL - 8000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails LDL ELISA Kit

Untere Nachweisgrenze: 6.32 pg/mL

Verwendungszweck: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human LDLR.

Analytische Methode: Quantitative

Spezifität: LDLR

Bestandteile:

• Plate
• Standard
• Diluent

Benötigtes Material: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Details zu LDL

Target: LDL (Low Density Lipoprotein (LDL))

Andere Bezeichnung: LDL

Hintergrund: Low-density lipoprotein (LDL) receptor (LDLR) is a mosaic protein of 839 amino acids which belongs to the LDLR gene family. It is a cell-surface receptor that recognizes the Apo B100, which is embedded in the outer phospholipid layer of LDL particles. The receptor also recognizes the Apo E protein found in chylomicron remnants and VLDL remnants.

Gen-ID: 3949

NCBI Accession: NM_000527

UniProt: P01130