ICAM1 ELISA Kit

Produktnummer ABIN4987078

Human ICAM1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human ICAM1.

Kurzübersicht für ICAM1 ELISA Kit (ABIN4987078)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Produktdetails ICAM1 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Analytische Methode: Quantitative

Spezifität: Natural and recombinant Human sICAM-1 Ligand

Sensitivität: 31 pg/mL

Benötigtes Material:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Anwendungsinformationen

Applikationshinweise: Detection Wavelength: 450 nm

Probenmenge: 20 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Andere Bezeichnung: sICAM-1

Substanzklasse: Viral Protein

Hintergrund: Intercellular Adhesion Molecule 1 (ICAM-1), also known as CD54, is a nearly ubiquitous transmembrane glycoprotein that plays a key role in leukocyte migration and activation (1, 2). Human ICAM-1 contains five Ig-like domains in its extracellular domain (ECD) and associates into non-covalently linked dimers (3, 4). Soluble forms of monomeric and dimeric ICAM-1 (sICAM-1) can be generated via proteolytic cleavage by cathepsin G, elastase, MMP-9, MMP-14/MT1-MMP, and TACE/ADAM17 (5 - 8). In the mouse, alternate splicing generates isoforms that lack particular Ig-like domains and are differentially sensitive to proteolysis (5). Within the ECD, human ICAM-1 shares 53 % amino acid sequence identity with mouse and rat ICAM-1.The principal binding partners of ICAM-1 are the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) (9 - 11). The multivalency of dimeric ICAM-1 increases its strength of interaction with LFA-1 (9, 10). ICAM-1 also binds several non-integrin ligands including CD43/sialophorin, fibrinogen, hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes (12 - 16). At sites of inflammation, ICAM-1 is upregulated on endothelial and epithelial cells where it mediates the adhesion and paracellular migration of leukocytes expressing activated LFA-1 and Mac-1 (17 - 20). ICAM-1 ligation prolongs antigen presentation by dendritic cells and promotes T cell proliferation and cytokine release (21 - 23). ICAM-1 activation also participates in angiogenesis, wound healing, and bone metabolism (24 - 26).Soluble ICAM-1 has been reported in serum, cerebrospinal fluid, urine, and bronchoalveolar lavage fluid (2, 27 - 31). Elevated levels of sICAM-1 in these fluids are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, abdominal fat mass, hypertension, liver disease, and certain malignancies (32 - 40). sICAM-1 promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage (41, 42). It also functions as an inhibitor of transmembrane ICAM-1 mediated activities such as monocyte adhesion to activated endothelial cells and sensitivity of tumor cells to NK cell-mediated lysis (7, 8).

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling