NAMPT ELISA Kit

Produktnummer ABIN4987044

Dieses Human NAMPT ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human NAMPT zu quantifizieren.

Kurzübersicht für NAMPT ELISA Kit (ABIN4987044)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Produktdetails NAMPT ELISA Kit

Untere Nachweisgrenze: 1.56 pg/mL

Analytische Methode: Quantitative

Spezifität: Natural and recombinant Human PBEF/ Visfatin Ligand

Sensitivität: 0.70 ng/mL

Benötigtes Material:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Anwendungsinformationen

Applikationshinweise: Detection Wavelength: 450 nm

Probenmenge: 20 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Andere Bezeichnung: PBEF/ Visfatin

Hintergrund: PBEF (preB cell colonyenhancing factor? also visfatin and nicotinamide phosphoribosyltransferase) is a 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the Cterminal 128 amino acids (aa 364491). Over aa 27491, human PBEF shares 96 % , 97 %, and 96 % aa identity with mouse, porcine, and canine PBEF, respectively.