IL-32 alpha ELISA Kit

Produktnummer ABIN4986954

Dieses Human IL-32 alpha ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IL-32 alpha zu quantifizieren.

Kurzübersicht für IL-32 alpha ELISA Kit (ABIN4986954)

Target: IL-32 alpha (IL32A) (Interleukin 32 alpha (IL32A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.625-1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Produktdetails IL-32 alpha ELISA Kit

Untere Nachweisgrenze: 15.625 pg/mL

Analytische Methode: Quantitative

Spezifität: Natural and recombinant Human IL-32a Ligand

Sensitivität: 7 pg/mL

Benötigtes Material:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Anwendungsinformationen

Applikationshinweise: Detection Wavelength: 450 nm

Probenmenge: 20 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IL-32 alpha

Target: IL-32 alpha (IL32A) (Interleukin 32 alpha (IL32A))

Andere Bezeichnung: IL-32a

Hintergrund: IL-32α is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. The IL-32α shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32α. Transfected IL-32α was more likely to be cellassociated as compared to IL-32β, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFNγ in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn induces cytokine expression.