IL-17 ELISA Kit

Produktnummer ABIN4986934

Human IL-17 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human IL-17 in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate) und Plasma (EDTA)

Kurzübersicht für IL-17 ELISA Kit (ABIN4986934)

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.625-1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Produktdetails IL-17 ELISA Kit

Untere Nachweisgrenze: 15.625 pg/mL

Analytische Methode: Quantitative

Spezifität: Natural and recombinant Human IL-17 Ligand

Sensitivität: 7 pg/mL

Benötigtes Material:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Anwendungsinformationen

Applikationshinweise: Detection Wavelength: 450 nm

Probenmenge: 20 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IL-17

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Andere Bezeichnung: IL-17

Hintergrund: IL-17, originally identified as mouse cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) (1), is produced by activated T lymphocytes, primarily by memory T cells (1-4). IL-17 appears to mediate communication between the immune system and the hematopoietic system.IL-17 is a disulfide-linked homodimer (2, 4). Each polypeptide has 155 amino acid (aa) residues (predicted mass = 17.5 kDa), including a 19 aa residue hydrophobic leader sequence (2). There are six cysteines plus one potential N-linked glycosylation site, which is variably glycosylated, at least with recombinant proteins (2, 4). The aa sequence of human IL-17 is 63 % and 58 % identical to mouse and rat IL-17 and 72 % identical to the thirteenth ORF of Herpesvirus saimiri (2, 4). There is at least some species specificity for in vitro action on bone-marrow stromal cells (3).IL-17 mediation of T cell communication with the hematopoietic system is suggested by two observations. T cell-derived IL-17 induces fibroblasts to produce IL-6, IL-8, ICAM-1 and G-CSF, apparently by an NF-?B-mediated mechanism (5). IL-6 in turn promotes development of granulocyte/macrophage colonies, and G-CSF directs development of neutrophils (4, 6-9). IL-17 also enhances proliferation of partially activated T cells (5) and upregulates nitric oxide (NO) production in osteoarthritic cartilage (10).