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Heparan sulfate (HS) ELISA Kit

Diverse Spezies ELISA-Kit Colorimetric-Assay zur Quantifizierung von Diverse Spezies .
Produktnummer ABIN4969780

Kurzübersicht für Heparan sulfate (HS) ELISA Kit (ABIN4969780)

Target

Heparan sulfate (HS)

Reaktivität

  • 4
  • 1
  • 1
Diverse Spezies

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

31.2 pg/mL - 2000 pg/mL

Applikation

ELISA

Proben

Plasma, Serum
  • Untere Nachweisgrenze

    31.2 pg/mL

    Verwendungszweck

    Heparan Sulfate (HS) ELISA Kit

    Analytische Methode

    Quantitative

    Sensitivität

    18.8 pg/mL

    Bestandteile

    The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
    • Pre-coated 96-Well Microplate
    • Standard
    • Standard Diluent Buffer
    • Wash Buffer
    • Detection Reagent A
    • Detection Reagent B
    • Diluent A
    • Diluent B
    • TMB Substrate
    • Stop Solution
    • Plate Sealer

    Benötigtes Material

    • 37 °C incubator
    • Multi and single channel pipettes and sterile pipette tips
    • Squirt bottle or automated microplate washer
    • 1.5 mL tubes
    • Distilled water
    • Absorbent filter papers
    • 100 mL and 1 liter graduated cylinders
    • Microplate reader (wavelength: 450 nm)
    • ELISA Shaker
  • Applikationshinweise

    Optimal dilutions/concentrations should be determined by the end user.

    Kommentare

    The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

    Plattentyp

    Pre-coated

    Testpräzision

    Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Heparan Sulfate (HS) were tested 20 times on one plate, respectively.

    Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Heparan Sulfate (HS) were tested on 3 different plates, 8 replicates in each plate.

    CV (%) = (Standard Deviation / mean) x 100

    Intra-Assay: CV<10%

    Inter-Assay: CV<10%

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C

    Informationen zur Lagerung

    Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

    Haltbarkeit

    6 months
  • Target

    Heparan sulfate (HS)

    Andere Bezeichnung

    Heparan Sulfate
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