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Angiotensin 1-7 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Angiotensin 1-7.
Produktnummer ABIN4967120

Kurzübersicht für Angiotensin 1-7 ELISA Kit (ABIN4967120)

Target

Alle Angiotensin 1-7 ELISA Kits anzeigen
Angiotensin 1-7

Reaktivität

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Human

Nachweismethode

Colorimetric

Methodentyp

Competition ELISA

Detektionsbereich

12.35 pg/mL - 1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
  • Untere Nachweisgrenze

    12.35 pg/mL

    Verwendungszweck

    Human Angiotensin 1-7 (Ang 1-7) ELISA Kit

    Analytische Methode

    Quantitative

    Sensitivität

    < 5.21 pg/mL

    Bestandteile

    The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
    • Pre-coated 96-Well Microplate
    • Standard
    • Standard Diluent Buffer
    • Wash Buffer
    • Detection Reagent A
    • Detection Reagent B
    • Diluent A
    • Diluent B
    • TMB Substrate
    • Stop Solution
    • Plate Sealer

    Benötigtes Material

    • 37 °C incubator
    • Multi and single channel pipettes and sterile pipette tips
    • Squirt bottle or automated microplate washer
    • 1.5 mL tubes
    • Distilled water
    • Absorbent filter papers
    • 100 mL and 1 liter graduated cylinders
    • Microplate reader (wavelength: 450 nm)
    • ELISA Shaker
  • Applikationshinweise

    Optimal dilutions/concentrations should be determined by the end user.

    Kommentare

    The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

    Probenmenge

    50 µL

    Plattentyp

    Pre-coated

    Aufbereitung der Reagenzien

    This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

    • 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
    • 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
    • 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.

    Testdurchführung

    This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

    • Equilibrate the kit components and samples to room temperature (18 - 25 °C) before use. It is recommended to plot a standard curve for each test.
    • 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate.
    • 2. Add 50 μL of each standard, control and sample into the appropriate wells.
    • 3. Remove the cover and discard the liquid.
    • 4. Immediately aliquot 50 μL of Detection Reagent A working solution. Seal the plate with a cover and incubate for 1 h at 37 °C.
    • 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer.
    • 6. Add 100 μL of Detection Reagent B working solution into each well, seal and incubate at 37 °C for 30 min.
    • 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step.
    • 8. Aliquot 90 μL of TMB Substrate into each well. Seal the plate with a cover and incubate at 37 °C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min.
    • 9. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.

    Ergebnisberechnung

    This assay is competitive, therefore there is an inverse correlation between Ang (1-7) concentration in the sample and the absorbance measured. Create a graph with the log of the standard concentration (y-axis) and average absorbance measured (x-axis). Apply a best fit trendline through the standard points. The Ang (1-7) concentration of the samples can be interpolated from the standard curve.

    Testpräzision

    Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Angiotensin 1-7 (Ang1-7) were tested 20 times on one plate, respectively.

    Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Angiotensin 1-7 (Ang1-7) were tested on 3 different plates, 8 replicates in each plate.

    CV (%) = (Standard Deviation / mean) x 100

    Intra-Assay: CV<10%

    Inter-Assay: CV<12%

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C

    Informationen zur Lagerung

    Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

    Haltbarkeit

    6 months
  • Target Alle Angiotensin 1-7 ELISA Kits anzeigen

    Angiotensin 1-7
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