Copeptin ELISA Kit

Produktnummer ABIN4889768

Das Human, Ratte und Maus Copeptin ELISA Kit (ABIN4889768) ist ein Colorimetric ELISA Kit zur Detektion von Human, Ratte und Maus Copeptin.

Kurzübersicht für Copeptin ELISA Kit (ABIN4889768)

Target: Copeptin (CPP)

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.1 ng/mL - 1000 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Copeptin ELISA Kit

Untere Nachweisgrenze: 0.1 ng/mL

Verwendungszweck: Human/Mouse/Rat Copeptin EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA

Analytische Methode: Quantitative

Spezifität: This EIA kit is designed to detect human, mouse, and rat copeptin and the propeptide Vasopressin-Neurophysin 2-copeptin.

Sensitivität: 0.5 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: Human: 2X / Mouse: 2X / Rat: 2X

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Aufbereitung der Reagenzien:

Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 8x / Rat: 8x

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Haltbarkeit: 6 months

Details zu Copeptin

Target: Copeptin (CPP)

Andere Bezeichnung: Copeptin

Hintergrund:

Gene Symbols: AVP, ARVP, VP

Background: Copeptin is a 39-amino acid peptide, mainly produced in the paraventricular neurons of the hypothalamus and in the supraoptical nucleus. It is cleaved from a protein also consisting of vasopressin and neurophysin II. Copeptin may help vasopressin folding during transportation. Vasopressin has a direct antidiuretic action on the kidney, and it also causes vasoconstriction of the peripheral vessels. Copeptin can be used as a vasopressin surrogate in clinical test. The clinical interest in copeptin testing is closely linked to the pathophysiological pathways in which vasopressin is involved, such as polydipsia-polyuria syndrome, hyponatremia, syndrome of inappropriate antidiuretic hormone secretion (SIADH) as well as heart failure and acute coronary syndrome.

Protein Name (Synonyms): Vasopressin-neurophysin 2-copeptin (AVP-NPII) [Cleaved into: Arg-vasopressin (Arginine-vasopressin), Neurophysin 2 (Neurophysin-II), Copeptin]

Gen-ID: 551

UniProt: P01185

Pathways: Hormone Activity