Adalimumab ELISA Kit

Produktnummer ABIN4886389

Das Human, Maus und Ratte Adalimumab ELISA Kit (ABIN4886389) ist ein Colorimetric ELISA Kit zur Detektion von Human, Maus und Ratte Adalimumab.

Kurzübersicht für Adalimumab ELISA Kit (ABIN4886389)

Target: Adalimumab

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 50 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Adalimumab ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Quantification of Adalimumab in biological matrices

Analytische Methode: Quantitative

Spezifität: Adalimumab (Humira)

Kreuzreaktivität (Details): hIgG1, rituximab, and infliximab prepared at 250 ng/mL were assayed and exhibited no crossreactivity or interference.

Sensitivität: 1.5 ng/mL

Produktmerkmale: The Adalimumab ELISA kit is designed to measure free Adalimumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Adalimumab molecules in biological matrices

Bestandteile:

• Coated microtiter plate, 96 wells
• Calibrator diluent. - 1.8ml
• Calibrator 12ul 10X wash buffer - 25ml
• Assay buffer - 50ml
• 1000X detection reagent - 17ul TMB - 12ml
• TMB stop solution - 12ml
• Plate sealers - 3

Benötigtes Material:

• Precision pipettes calibrated to deliver 5-1000μL
• Multi-channel pipette calibrated to deliver 50-200μL
• Plate shaker
• Disposable tips
• Vortex-Mixe
• Distilled or de-ionized water
• Microplate reader capable of reading 450nm with background subtraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 15 μL

Testdauer: 2.5 h

Plattentyp: Pre-coated

Protokoll: The Adalimumab ELISA kit is designed to measure free Adalimumab with high specificity and sensitivity. This assay employs the sandwich enzyme immunoassay technique. A precoated anti-Adalimumab 96 well plate is provided. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Adalimumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Adaliumumab present in test samples and the concentration is calculated from the standard series.

Aufbereitung der Reagenzien:

Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Wash Buffer (1X) Preparation: Dilute wash buffer concentrate with ultra-pure water 1/10 before use (for example add 20 mL concentrate to 180 mL ultra-pure water). Mix well. 2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 11 μL concentrate to 11 mL of assay buffer). Mix well. 3. Preparation of Calibrators: Prepare calibrators with concentrations ranging from 2,500 ng/mL to 78 ng/mL. The following is an example calibrator curve.

Probennahme: This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20 °C for up to 1 year.

Aufbereitung der Proben:

Dilute calibrators and test samples 1/50 with assay buffer (for example add 5μL of prepared calibrator or sample to 245μL of assay buffer). Mix well. Do not store diluted samples.

Testdurchführung:

This assay employs the sandwich enzyme immunoassay technique. Anti- Adalimumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Adalimumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Adalimumab present in test samples.

Ergebnisberechnung:

1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. Alternatively a log-log curve fit may be used. 2. The concentration of the unknowns can be read directly from this standard curve using the absorbance value for each sample. 3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with calibrator diluent and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor.

Testpräzision: Intra-assay precision: <10%
Inter-assay precision: <10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: -20C, 1 year

Haltbarkeit: 12 months

Details zu Adalimumab

Target: Adalimumab

Hintergrund: Adalimumab (Trade name Humira®) is a human monoclonal antibody used to block the action of tumor necrosis factor alpha (TNF- ). TNF-α is a strong proinflammatory cytokine involved in normal immunity and also various autoimmune diseases. The Adalimumab ELISA kit is designed to measure free Adalimumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Adalimumab molecules in biological matrices

Gen-ID: 7124