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TNK1 ELISA Kit

Dieses Human TNK1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human TNK1 zu quantifizieren.
Produktnummer ABIN4886327

Kurzübersicht für TNK1 ELISA Kit (ABIN4886327)

Target

Alle TNK1 ELISA Kits anzeigen
TNK1 (Tyrosine Kinase, Non-Receptor, 1 (TNK1))

Bindungsspezifität

pTyr

Reaktivität

  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Cell Lysate, Tissue Lysate
  • Verwendungszweck

    Custom Human Phosphotyrosine TNK1 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine TNK1 in lysate samples.

    Analytische Methode

    Semi-Quantitative

    Spezifität

    The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-TNK1.

    Produktmerkmale

    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

    Haltbarkeit

    6 months
  • Target Alle TNK1 ELISA Kits anzeigen

    TNK1 (Tyrosine Kinase, Non-Receptor, 1 (TNK1))

    Andere Bezeichnung

    TNK1

    Gen-ID

    8711

    UniProt

    Q13470
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