ERK1, JNK, P38 ELISA Kit

Produktnummer ABIN4886189

Das Human, Maus und Ratte ERK1, JNK, P38 ELISA Kit (ABIN4886189) ist ein Colorimetric ELISA Kit zur Detektion von Human, Maus und Ratte ERK1, JNK, P38. Das Kit wurde in 2 Publikationen zitiert.

Kurzübersicht für ERK1, JNK, P38 ELISA Kit (ABIN4886189)

Target: ERK1, JNK, P38

Bindungsspezifität: pThr180, pThr183, pThr202, pTyr182, pTyr185, pTyr204

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Lysate, Tissue Lysate

Produktdetails

Verwendungszweck: Phospho-ERK/JNK/P38? ELISA Kit. This assay semi-quantitatively measures phophorylated ERK/JNK/P38? in lysate samples.

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes ERK1/2 (T202/Y204), JNK (T183/Y185), P38α (T180/Y182), Total ERK1/2, JNK and P38α in Human, Mouse and Rat Cell Lysates.

Produktmerkmale:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Referenzen

Elamin, Masclee, Troost, Pieters, Keszthelyi, Aleksa, Dekker, Jonkers: "Ethanol impairs intestinal barrier function in humans through mitogen activated protein kinase signaling: a combined in vivo and in vitro approach." in: PLoS ONE, Vol. 9, Issue 9, pp. e107421, (2015) (PubMed).

Elsherbiny, Abd El Galil, Gabr, Al-Gayyar, Eissa, El-Shishtawy: "Reno-protective effect of NECA in diabetic nephropathy: implication of IL-18 and ICAM-1." in: European cytokine network, Vol. 23, Issue 3, pp. 78-86, (2012) (PubMed).

Antigendetails

Target: ERK1, JNK, P38

Andere Bezeichnung: ERK1, JNK , P38