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SIGLEC5 ELISA Kit

SIGLEC5 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4885336
  • Target Alle SIGLEC5 ELISA Kits anzeigen
    SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
    Reaktivität
    • 4
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Siglec-5 (CD170) SpeedELISA Kit.
    Marke
    SpeedELISA
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Compressed workflow with only one wash step and three incubation steps
    • 3 hours total processing time
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Streptavidin-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Capture Antibody
    • HRP Conjugated Detection Antibody
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product SIGLEC5 ELISA Kit
  • Probenmenge
    100 μL
    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 70 μL of the Antibody Cocktail to each well.
    3. Incubate 10 minutes at RT.
    4. Add 50 μL of sample or standard to each well.
    5. Incubate 2 h at RT.
    6. Wash Wells
    7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    8. Incubate 30 min at RT.
    9. Add 50 μL of Stop Solution to each well.
    10. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
    Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    6 months
  • Target Alle SIGLEC5 ELISA Kits anzeigen
    SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
    Andere Bezeichnung
    Siglec-5 (SIGLEC5 Produkte)
    Synonyme
    CD170 ELISA Kit, CD33L2 ELISA Kit, OB-BP2 ELISA Kit, OBBP2 ELISA Kit, SIGLEC-5 ELISA Kit, sialic acid binding Ig like lectin 5 ELISA Kit, sialic acid-binding Ig-like lectin 5 ELISA Kit, sialic acid binding Ig-like lectin 5 ELISA Kit, SIGLEC5 ELISA Kit, LOC100597560 ELISA Kit, Siglec5 ELISA Kit
    Gen-ID
    8778
    UniProt
    O15389
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