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DKK3 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human DKK3.
Produktnummer ABIN4885192

Kurzübersicht für DKK3 ELISA Kit (ABIN4885192)

Target

Alle DKK3 ELISA Kits anzeigen
DKK3

Reaktivität

  • 4
  • 4
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Plasma, Cell Culture Supernatant, Serum
  • Verwendungszweck

    Custom Human DKK-3 SpeedELISA Kit.

    Marke

    SpeedELISA

    Analytische Methode

    Quantitative

    Produktmerkmale

    • Compressed workflow with only one wash step and three incubation steps
    • 3 hours total processing time
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Streptavidin-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Capture Antibody
    • HRP Conjugated Detection Antibody
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge

    100 μL

    Testdauer

    3 h

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 70 μL of the Antibody Cocktail to each well.
    3. Incubate 10 minutes at RT.
    4. Add 50 μL of sample or standard to each well.
    5. Incubate 2 h at RT.
    6. Wash Wells
    7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    8. Incubate 30 min at RT.
    9. Add 50 μL of Stop Solution to each well.
    10. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
    Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    6 months
  • Target Alle DKK3 ELISA Kits anzeigen

    DKK3

    Andere Bezeichnung

    DKK-3

    Gen-ID

    27122

    UniProt

    Q9UBP4
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