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NAMPT ELISA Kit
NAMPT
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4885043
Produktdetails NAMPT ELISA Kit
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Target
Alle NAMPT ELISA Kits anzeigen
NAMPT
(Nicotinamide phosphoribosyltransferase (NAMPT))
Reaktivität
Alle Reaktivitäten für NAMPT ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Visfatin ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes .
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu NAMPT
(ausblenden)
Target
Alle NAMPT ELISA Kits anzeigen
NAMPT
(Nicotinamide phosphoribosyltransferase (NAMPT))
Andere Bezeichnung
Visfatin (NAMPT Produkte )
Synonyme
1110035O14Rik ELISA Kit, PBEF ELISA Kit, PBEF1 ELISA Kit, VF ELISA Kit, VISFATIN ELISA Kit, AI314458 ELISA Kit, AI480535 ELISA Kit, NAmPRTase ELISA Kit, Pbef ELISA Kit, Pbef1 ELISA Kit, Visfatin ELISA Kit, visfatin ELISA Kit, pbef ELISA Kit, pbef1 ELISA Kit, nicotinamide phosphoribosyltransferase ELISA Kit, Nicotinamide phosphoribosyltransferase ELISA Kit, nicotinamide phosphoribosyltransferase S homeolog ELISA Kit, NAMPT ELISA Kit, Nampt ELISA Kit, nampt ELISA Kit, Smon_0680 ELISA Kit, Mrub_2845 ELISA Kit, Cseg_3704 ELISA Kit, BC1002_3997 ELISA Kit, Ftrac_2686 ELISA Kit, Varpa_5681 ELISA Kit, Celal_3900 ELISA Kit, Deima_0880 ELISA Kit, BC1001_3724 ELISA Kit, Celly_1696 ELISA Kit, Clole_1542 ELISA Kit, Tsp_05934 ELISA Kit, nampt.S ELISA Kit
Gen-ID
10135
UniProt
P43490
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