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UGT1A1 ELISA Kit

UGT1A1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884976
  • Target Alle UGT1A1 ELISA Kits anzeigen
    UGT1A1 (UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1))
    Reaktivität
    • 3
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human UGT1A1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes .
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle UGT1A1 ELISA Kits anzeigen
    UGT1A1 (UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1))
    Andere Bezeichnung
    UGT1A1 (UGT1A1 Produkte)
    Synonyme
    BILIQTL1 ELISA Kit, GNT1 ELISA Kit, HUG-BR1 ELISA Kit, UDPGT ELISA Kit, UDPGT 1-1 ELISA Kit, UGT1 ELISA Kit, UGT1A ELISA Kit, Gnt1 ELISA Kit, UGT1A01 ELISA Kit, Udpgt-1a ELISA Kit, UgtBr1 ELISA Kit, Udpgt ELISA Kit, Ugt1 ELISA Kit, zgc:123097 ELISA Kit, UDP glucuronosyltransferase family 1 member A1 ELISA Kit, UDP glucuronosyltransferase 1 family, polypeptide A1 ELISA Kit, UDP-glucuronosyltransferase 1-1 ELISA Kit, UDP-glucuronosyltransferase ELISA Kit, UDP-glucuronosyltransferase 1-6 ELISA Kit, UDP glucuronosyltransferase 1 family polypeptide a1 ELISA Kit, UGT1A1 ELISA Kit, Ugt1a1 ELISA Kit, LOC100065342 ELISA Kit, LOC100125517 ELISA Kit, LOC100229734 ELISA Kit, LOC100405984 ELISA Kit, LOC100511479 ELISA Kit, ugt1a1 ELISA Kit
    Gen-ID
    54658
    UniProt
    P22309
    Pathways
    Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha
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