Transferrin Receptor ELISA Kit

Produktnummer ABIN4884756

Produktdetails Transferrin Receptor ELISA Kit

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.819-200 ng/mL

Untere Nachweisgrenze: 0.819 ng/mL

Applikation: ELISA

Verwendungszweck: Human TfR/Transferrin R/CD71 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human TfR.

Sensitivität: 0.81 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples200 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Andere Bezeichnung: TfR (Transferrin R/CD71) (TFRC Produkte)

Synonyme: Trfr ELISA Kit, LOC100219280 ELISA Kit, tfr ELISA Kit, cd71 ELISA Kit, tfr1 ELISA Kit, trfr ELISA Kit, CD71 ELISA Kit, T9 ELISA Kit, TFR ELISA Kit, TFR1 ELISA Kit, TR ELISA Kit, TRFR ELISA Kit, p90 ELISA Kit, 2610028K12Rik ELISA Kit, AI195355 ELISA Kit, AI426448 ELISA Kit, AU015758 ELISA Kit, E430033M20Rik ELISA Kit, Mtvr-1 ELISA Kit, Mtvr1 ELISA Kit, transferrin receptor ELISA Kit, TFRC ELISA Kit, Tfrc ELISA Kit, tfrc ELISA Kit

Gen-ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis